Affinity chromatography has a long history of use in biopharmaceutical manufacturing and is a powerful tool in making industrial processes more efficient by reducing the number of steps required to achieve the desired target purity and yield of a final product. It is common, however, for teams to consider affinity chromatography once all other options have been considered, evaluated, and discarded. Such an approach usually translates into a need to develop affinity adsorbents for new targets quickly, efficiently, and economically.

This article outlines the steps required to develop an affinity adsorbent, including the identification of ligands, choice of base matrices, attachment chemistries, use of spacer arms, and other considerations relevant to the development of novel affinity adsorbents for use in biopharmaceutical manufacture.

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