Figure 1: High-resolution separation of pDNA from ssRNA; double-stranded dsRNA also elutes in advance of ssRNA.

CIMmultus PrimaS multimode ligand bioprocessing technology represents a new class of anion exchangers for fast, efficient, one-step purification of single-stranded mRNA at ambient temperature. DNA, dsRNA, and proteins are eliminated with a high-salt wash. Then ssRNA is fractionated by size in a pH gradient that removes short transcripts and fragments. Convective mass transfer and laminar flow through the technology’s monolithic architecture maintain high capacity, high resolution, and low shear stress — even at flow rates >5 column volumes per minute.

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