Author Archives: Esohe E. Idusogie Joni M. Castro Clayton Casipit Aaron Sato Yuko Terasawa Michael G. Mulkerrin

Development of an Antibody Screening Assay for Selection of Production Cell Lines

Biopharmaceuticals such as recombinant monoclonal antibodies (MAbs) account for a significant proportion of all new drugs (1,2,3). Although manufacturing site capacities have expanded and process efficiencies have improved greatly, there is still some concern the current biomanufacturing capacity worldwide might not meet increasing market demands (1, 2). PRODUCT FOCUS: RECOMBINANT PROTEINSPROCESS FOCUS: PRODUCTION PROCESS DEVELOPMENTWHO SHOULD READ: QA/QC, PROCESS DEVELOPMENT, AND ANALYTICAL PERSONNELKEYWORDS: CELL LINE DEVELOPMENT, SCREENING ASSAY, MONOCLONAL ANTIBODIES, FC PROTEINS, CHO, IGG, HTRF, ANTIBODY TITER, CELL LINE PRODUCTIVITY,…