Efficient expression of proteins is an integral part of the biopharmaceutical drug development process. With a range of expression systems, it is important to balance long-term manufacturing goals (e.g., cost of goods and productivity) while matching the expression system to suit the characteristics of a protein (e.g., product quality attributes and long-term stability). Finding the right expression system early on can make or break a therapeutic candidate as it moves from laboratory bench to clinical administration.
FUJIFILM Diosynth Biotechnologies has developed its pAVEwayâ„¢ expression technology to support microbial expression of proteins. This platform technology incorporates three elements in the delivery of an Escherichia coli expression process: optimized expression vectors and control elements, preselected host strains, and platform development using a high-throughput approach for fast strain selection and/or design of experiment (DoE) approaches.
DNA looping in pAVEwayâ„¢ expression technology is key for controlled, reproducible, and scalable protein expression.
Figure 1: DNA loop formation mechanism that helps control on–off protein expression
DNA Looping and Control Elements