Boehringer Ingelheim stands out due to global contract manufacturing excellence in plasmid DNA production. It is one of the world’s leading companies for contract development and manufacturing of biopharmaceuticals
Author Archives: BPI Contributor
Applying Single-Use Efficiencies to Room-to-Room Fluid Transfer
This report explains how a wall pass-through system that incorporates Single-Use components can help speed and streamline operations, reduce contamination risks and maintain isolated environments.
Parallel Cultivation of Microorganisms Using Rigid Wall Single-Use Bioreactors
This webcast features: Dr. Sebastian Kleebank, development and application lab manager, DASGIP GmbH (part of Eppendorf AG) Single-use bioreactor solutions have been successfully established in animal and human cell culture in the last years. Currently this technology is investigated for microbial applications, especially for high cell density fermentations. Dr. Sebastian Kleebank demonstrates that reproducible process control could be achieved using single-use bioreactors operated in parallel.
Defining Non-Animal Derived Supplements for the Optimization of Mammalian Cell Culture Media Using DoE Methodologies
Optimization of chemically defined animal-derived component free media to culture mammalian cells and optimization of existing commercial media is possible with their supplementation with specific additives.
Efficacy and Utility of New EXP-Pak Closed-System Disposable Cell Expansion Bags Designed for Cell Therapy Applications
Clinical cellular therapy applications often times require a cell expansion or maturation step prior to use. Traditionally, cell expansion or cell culture is performed in “open†systems including multi-well culture dishes or tissue culture flasks. These “open†steps present risks and are not ideal for larger-scale manufacturing. The new EXP-Pakâ„¢ cell expansion container offered by Charter Medical, Ltd. is a closed-system, gas permeable bag intended for expansion and culture of non-adherent cells. The data demonstrate that the EXP-Pakâ„¢ cell expansion…
Freeze-Pak™ STS Bio-Containers and STS Shippers: Offering a New Single-Use Solution for Frozen Storage and Transport to -80°C
Frozen storage is commonly achieved with single-use containers and enables manufacturing process flexibility, long-term product stability and minimizes logistics challenges. While single-use containers are available for storage and transport of frozen products, some require a significant investment while others don’t offer the necessary support and protection. Charter Medical has recently developed a new single-use frozen storage and transport shipping solution designed to complement the single-use Freeze-Pakâ„¢ STS (FP-STS) bio-containers which were engineered specifically for frozen storage applications. The bags and…
New Sensitive Amp’d™ GLP-1 ELISA Kit for the Rapid Detection of Glucagon-Like Peptide 1
Strengthening our leadership position in immunoassays, Enzo Life Sciences now offers Amp’dâ„¢ GLP-1 ELISA Kit – a rapid ELISA for the detection of the active form of Glucagon-Like Peptide 1. GLP-1 functions in the regulation of blood glucose through insulin, and plays a role in obesity and diabetes. It is the most potent promoter of insulin secretion and an important gastrointestinal peptide hormone, or incretin. In response to eating, increased levels of GLP-1 stimulate insulin secretion and inhibit glucagon release leading…
Ready-to-Use EZ-Fitâ„¢ Filtration Unit from EMD Millipore Leverages Industry Leading Technology for More Efficient Bioburden Testing of Liquids
Reduces risk of sample contamination associated with filtration and membrane transfer Accelerates filtration process and delivers more reliable results Completes comprehensive product portfolio enabling more efficient bioburden analysis and microbial detection July 30, 2014, Billerica, MA – EMD Millipore, the Life Science division of Merck KGaA of Darmstadt, Germany, has launched the EZ-Fitâ„¢ Filtration Unit, a ready-to-use, disposable filtration device for bioburden testing. The system is more efficient than traditional methods, resulting in increased reliability and streamlined workflows. The unit, which conforms…
Evaluation of HCP and DNA Clearance with NatriFloâ„¢ HD-Q Membrane Adsorbers at Lab to Process Scales
NatriFloâ„¢ HD-Q chromatography from Natrix Separations introduces reliable and reproducible Q polish performance that combines resin binding capacity with adsorber speed to achieve a new level of process flexibility. Gallus Biopharmaceuticals (a St. Louis based CMO) evaluated NatriFloâ„¢ HD-Q membrane columns as an anion exchange polishing step with the objective of assessing NatriFlo’s ability to (1) reduce process-derived impurities to achieve target product profile; (2) maintain process robustness to process changes; and (3) achieve reliable scalability from lab to production.…
Use and Optimization of Chemically Defined Media Supplements in Mammalian Cell Culture Processes
The manufacture of biological therapeutics requires a recombinant cell line, a media system and process to produce the therapeutic, and a purification process. BD Advanced Bioprocessing specializes in the media system to produce the therapeutic, including cell culture media and a variety of different types of supplementation.There are many types of supplementation, from animal-origin and animal-free hydrolysates to chemically defined, and each biopharma needs to find the best supplementation for their systems’ requirements.
Chemically defined media supplementation has many benefits such as lot-to-lot consistency and reduction/elimination of the risk of animal-origin materials. However, to get optimal performance these supplements are not always as forgiving as a full-bodied hydrolysate.
This webinar will use the newly commercialized BD CD Resurgeâ„¢ supplements as a case example on how to use and optimize CD supplements in mammalian cell culture processes.