Author Archives: BPI Contributor

New Device for Biomass Monitoring in Shake Flask Culture

by

We developed a novel system for online biomass measurement in shake flasks based on near 360° scattered light detection. The optosensoric components were combined with the established PreSens SFR Shake Flask Reader technology, so biomass, pO2 and pH can be monitored non-invasively through the flask bottom. First application of the prototype SFR vario on Escherichia coli K12 and Kluyveromyces marxianus culture resulted in highly reproducible online biomass data, with relative errors matching those of standard offline OD measurements.

May 27, 2015

VTU Technology and Biomay Sign License Agreement for Pichia pastoris Technology

by

VTU Technology and Biomay, two leading Austrian biotech companies, announced today, that they have entered into a licensing agreement for the production of recombinant allergens. VTU Technology, currently offering a very broad and comprehensive Pichia pastoris expression platform, grants Biomay access to VTU´s Pichia pastoris protein expression technology. Biomay, a global leader in allergy immunotherapy, will apply industrial Pichia expression strains generated by VTU for the commercial production of recombinant allergens. Under the terms of the agreement, VTU Technology will…

May 20, 2015

CaPure-HAâ„¢: Purification of MAb Fragments

by

Making use of chromatography resins with better selectivity, resolution and capacity is one approach to solving the problem not only of aggregate removal, but also that of MAb fragment and other digest products, in a column chromatography purification step. The data presented here demonstrate the capabilities of CaPure-HA to separate Fab and Fc fragments from undigested MAb in a papain digest of an IgG1 monoclonal antibody.

May 18, 2015

ZenBio, Inc. Introduces GroProâ„¢ Cell Culture Growth Supplement

by

ZenBio, Inc. industry leader in robust cell-based solutions, announced today the worldwide release of a new GroProâ„¢ Cell Culture Growth Supplement. The GroProâ„¢ product line allows for culturing of primary cells and cell lines without the concern for transmission of bovine prions and xenogeneic reactions. “The new GroPro line delivers higher standards of purity, more affordable cell culture solutions in biomedical research,” said Dr. John W. Ludlow, Executive Director of Regenerative Medicine at ZenBio “Our goal was to formulate a…

May 14, 2015

Goodwin Biotechnology Selected by Panacea Pharmaceuticals for Highly Specialized Vaccine Fill and Finish Project

by

Goodwin Biotechnology, Inc., a biological contract development and manufacturing organization (CDMO) that specializes in bioprocess development and GMP manufacturing of biopharmaceuticals utilizing mammalian cell culture expression systems and bioconjugation technologies, was selected by Panacea Pharmaceuticals, Inc. to complete a novel fill and finish project, as well as quality control release and stability testing for a therapeutic, nanoparticle cancer vaccine based on the Human Aspartyl (Asparaginyl) β-Hydroxylase (HAAH) tumor-specific protein to support phase 1 clinical trials in patients with various solid…

May 11, 2015

Cadenceâ„¢ Single-Pass TFF Coupled with Chromatography Steps Enables Continuous Bioprocessing While Reducing Processing Times and Volumes

by

Continuous bioprocessing initiatives have accelerated in recent years. The transition from batch to continuous bioprocessing offers numerous advantages, including process time savings and lower capital costs due to smaller equipment, tanks, and tubing sizes. Additionally, hold tanks may no longer be needed between unit operations, which can be especially valuable for facilities with limited manufacturing floor space. Furthermore, reduced system hold-up volumes improve product recoveries and also contribute to the smaller system footprint of continuous bioprocessing systems. One unit of…

May 8, 2015

Volume Reduction and Process Optimization with Cadenceâ„¢ Inline Concentrator

by

Pall’s Cadenceâ„¢ single-pass tangential flow filtration (SPTFF) technology provides a simple solution for continuous concentration and optimization of Downstream Processing (DSP) steps. It eliminates the conventional TFF recirculation loop and allows product to be concentrated in a single pump pass. The single-pass operation eliminates any mixing or foaming issues and exposes the product to low shear; thus, it is optimal for the processing of fragile and shear-sensitive molecules as well. The single-pass feed flow rates are lower compared to conventional…

May 8, 2015

Nikon and Lonza Form Collaboration for Cell and Gene Therapy Manufacturing in Japan

by

Lonza, the world’s leading developer and manufacturer of cells for regenerative medicine therapeutics, and Nikon Corporation announced today an exclusive collaboration in the field of cell and gene therapy manufacturing in Japan. Nikon will have access to Lonza’s quality and operating systems, facility design and ongoing consulting services for the establishment of a wholly owned Nikon cell and gene therapy contract manufacturing business. The Tokyo/Yokohama area of Japan is currently under consideration for the site location of the Nikon’s new…

May 7, 2015

Lucigen Corporation Expands Technological Reach With Exclusive Sigma-Aldrich Distribution Agreement

by

Lucigen Corporation announced today that it has signed an exclusive distributorship agreement with Sigma-Aldrich Corporation’s (NASDAQ: SIAL) India subsidiary, Sigma-Aldrich Chemicals Private Ltd., to expand commercialization of life science reagents in India. The synergy between the two companies will provide researchers in India an enhanced customer experience, strong technical support, and easy access to Lucigen’s broad portfolio of products and services. Ralph Kauten, CEO & Chairman of the Board for Lucigen Corporation, noted, “Sigma-Aldrich’s market knowledge and logistics operations in…

May 6, 2015

Design of a New Range of High Capacity and High Flow Agarose Resins

by

With the continuing development of high titer cell lines, resins with very high capacity at relevant residence times are becoming increasingly important. This paper will present application and characterization data from a set of novel, agarose-based resins, with ion exchange functionality as well as with immobilized alkaline stable Protein A. The cation exchanger has a dynamic binding capacity of more than 120 mg monoclonal antibody/mL resin without the introduction of surface extenders. Performance data from an alkaline stable Protein A…

May 6, 2015