Author Archives: James Furey

Tools For Early-Stage Purification Development of Filtration Operations

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Detailed early-stage development of filtration unit operations is critical to achieving a robust and reliable full-scale process. Despite the importance of this type of development work, fully evaluating a specific unit operation often can be difficult because of time constraints and a lack of material available for experimentation. As a result, a laboratory must have the proper equipment required to conduct efficient, high-throughput experiments. PendoTECH® has developed the ideal tools to address these types of challenges and enable laboratories to…

August 3, 2019

Using In-Line Disposable Pressure Sensors to Evaluate Depth Filter Performance

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    Development of a recovery process for a fed-batch mammalian cell culture product involves several objectives: process scalability, robustness, maximizing product yield, elimination of subsequent purification steps, and low cost of goods. In an effort to achieve those objectives, we developed a three-stage primary recovery process to remove biomass and clarify the feed stream for downstream column chromatography (Figure 1). The initial stage involves removal of whole cells and larger cellular debris using a continuous disc-stack centrifuge. Depth filtration…

February 1, 2010