with Dr. Laura Juckem High-efficiency transient transfections of suspension- adapted Chinese hamster ovary (CHO) cells enable researchers to bridge upstream and downstream processes. This helps to provide confidence that a recombinant protein will have the same properties throughout its development as a biologic. As drug targets become increasingly complex, proper protein folding and posttranslational modifications become more relevant. Early upstream work with the same cell type — e.g., CHO instead of HEK 293 cells — ensures similar product quality from…
Thursday, January 8, 2015 Daily Archives
Parallel Cultivation of Microorganisms – Using Rigid-Wall Single-Use Bioreactors
with Dr. Sebastian Kleebank In recent years, single-use bioreactor solutions have been successfully established for animal and human cell culture processes. Such technology is under investigation for microbial applications. Sebastian Kleebank is development and application laboratory manager for the Eppendorf line of bioprocess products. His cell culture and microbial laboratory performs parallel cultivations for prototype testing, user training, and software development. Kleebank’s Presentation “The BioBLU 1f bioreactor has a gas-inlet filter for sterile gas supply. It has a fully featured…