Eshmunoâ„¢ is a new and unique family of ion-exchange resins that are designed specifically for highly productive downstream bioprocessing. The first member of the family, Eshmunoâ„¢ S, is a smart cation exchanger designed for fast and efficient purification of antibodies in direct capture and steps following protein A chromatography. Eshmunoâ„¢ Q is the newest family member. This smart anion-exchange resin coupled Merck KGaA’s renowned tentacle structure with the new hydrophilic polyvinyl ether base matrix. As a result, Eshmunoâ„¢ Q offers outstanding results in typical anion-exchange applications, such as removing impurities in flow-through mode or separating blood factors in plasma processing.
Superior Productivity for Biomolecule Purification
Eshmunoâ„¢ Q exhibits a superior binding capacity for various biomolecules. Figure 1 shows the dynamic binding capacity (DBC) of Eshmunoâ„¢ Q for selected macromolecules at different flow rates. High flow rates (2 minutes residence time correspond to ~600 cm/hour) do not affect the high binding capacities at low flow rates.
More Stability
Unlike conventional anion-exchange resins, Eshmunoâ„¢ Q is intrinsically stable against alkaline solutions used in column sanitization. Figure 2 shows the increased stability of Eshmunoâ„¢ Q during storage in 1 M sodium hydroxide solution. Eshmunoâ„¢ Q retains its high binding capacity, whereas other anion-exchange resins suffer considerable reductions.
Tangible Savings in Cost and Development Time
Eshmunoâ„¢ Q showed remarkably sharp elution of the target molecule in a wide range of applications. Figure 3 shows the elution profile of purified MAb03 at pH 9.5 with 2 minutes residence time (CV = 1 mL), compared with the profile of another surface-grafted anion exchanger. Eshmunoâ„¢ Q elutes the antibody in a sharp peak of 12.4 mL; the other resin’s eluate volume is 27.6 mL.
The 130% higher concentration of the target molecule and the 60% reduced elution volume render further concentration steps unnecessary, saving money on buffer solutions and consumables. Our Global Applied Technology Department supports Eshmunoâ„¢ users with a global network of applications centers on three continents and with renowned training programs.
Author Details
Jessica Schrampf is marketing manager for LSS, 480 South Democrat Rd, Gibbstown NJ 08027; 1-856-423-6300; jessica.schrampf@emdchemicals.com; www.emdchemicals.com.