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Evaluation of Rapid Quantitation Methods for Titer, HCP and ProA By Gyrolab Platform During In-Process Development

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Getting biopharmaceuticals through development and into clinical proof-of-concept fast and efficiently is critical for success in our industry. Therefore, high-throughput upstream and downstream process development approaches are increasingly being implemented. Hence, innovative and high-throughput analytical technologies are needed to support rapid process development. A novel automated analytical platform-Gyrolab xP workstation was investigated for the analysis of IgG titer, Host Cell Protein (HCP) and leached ProA during in-process development. The assays are automated within a Gyrolab compact disc (CD) containing affinity-capture…

November 10, 2014

Optimization of HEK 293 and CHO Cell Culture Media Supplementation by DoE Methodology

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This webcast features: Dr. Francesc Gòdia, Professor of Chemical Engineering at Universitat Autònoma de Barcelona Chemically defined and animal derived free components media are required for bioprocess development and operation using mammalian cell as platform for the production of biopharmaceuticals. The supplementation of commercial chemically-defined media with specific compounds is studied for the optimization of CHO and HEK 293 cell culture. Design of Experiments approach is used to first screen the most efficient compounds and further determine their optimal conditions.…

October 29, 2014

CaPure-HA Resin for Aggregate Removal from Monoclonal Antibodies

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Scientists in the field of process chromatography methods development are constantly on the lookout for better and more selective ways to remove aggregates and other process related impurities from monoclonal antibody monomer. The data presented in the following paper demonstrates the capabilities of CaPure-HA to remove degradation products, dimer, and higher order aggregates from the monomer of a protein A purified IgG1 monoclonal antibody.

October 20, 2014

Your Shake Flask is not a Black Box Anymore! Online Measurements of Metabolic Activity Accelerate Your Bioprocess Development

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This webcast features: Dr. Gernot Thomas John, director of marketing and innovation at PreSens Precision Sensing GmbH Shake Flasks are widely used for bioprocess development. The most widely measured parameter to determine the biomass is the optical density OD. Typically, this parameter is measured not continuously and offline. Now PreSens is launching the new SFR vario. This compact device is placed underneath the shake flasks and measures 4 parameters online: pH, Oxygen, Oxygen consumption (OUR) and biomass. Join GT as…

October 15, 2014

Multitron Pro: The Professional Incubation Shaker for Any Application

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The Multitron Pro and the Multitron Cell manage to combine flexibility, user-friendliness and operational reliability with  optimum space utilization. A number of innovative options for cooling, precise CO2 control, illumination and hygienic direct steam humidification guarantee the ideal conditions for cultures. With around 500 different designs we meet almost every customer requirement. We are also more than happy to provide special configurations. INFORS HT provides you with Swiss quality with a modern design and a high technological level and environmentally friendly energy…

October 10, 2014

Increase Protein Yield through Optimization of Suspension CHO Transient Transfection

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This webcast features: Laura Juckem, PhD, R&D Group Leader, Mirus Bio High efficiency transient transfections of suspension CHO cells enable researchers to bridge upstream and downstream processes and provide confidence that recombinant protein will have the same properties throughout the drug discovery pipeline. To address this need, Mirus Bio has developed the TransIT-PRO® Transfection Kit to maximize plasmid DNA delivery and therefore recombinant protein yield. There are several important parameters to consider during transient transfection including: (1) growth media compatibility,…

October 2, 2014

Scalable Protein Production Using Flow Electroporation

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Many transient gene expression (TGE) methods produce insufficient protein quantities for full use within biotherapeutic and vaccine development pipelines. MaxCyte’s proprietary flow electroporation provides a universal means of highly efficient TGE for the rapid production of large-scale quantities of proteins, antibodies, antibody-like molecules, virus-like particles (VLPs), and vaccines. In addition, the platform can be used for generation of stable pools and stable cell lines that can greatly streamline biotherapeutic and vaccine development. Flow electroporation (co)transfects a wide range of cells,…

September 26, 2014