This application note describes the cultivation of Chinese hamster ovary (CHO) suspension cells in the Finesse SmartGlass vessel bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 106 cells/mL were achieved. Recombinant secreted embryonic alkaline phosphatase (SEAP) expression was induced by medium exchange and temperature shift. Maximum SEAP activities of up to 63 U/mL were reached. A novel stirred glass bioreactor suitable for cell culture applications at benchtop scale…

Cell culture is widely used for stable expression of monoclonal antibodies with posttranslational modifications. Despite having a stable cell line, a protein product is still in danger of improper modifications from chemical interactions with media. For example, the primary amino groups on a protein are susceptible to reactions with reducing sugar molecules. In the case of a reaction with glucose, this is called glycation. The relationship between glucose concentration and glycation is explored here. Methods: Chinese hamster ovary (CHO) cells…

Frozen storage is commonly achieved using single-use containers that enable manufacturing process flexibility and long-term product stability as well as minimize logistics challenges. Single-use containers also are available for storing and transporting frozen products although some require a significant investment, and others don’t offer necessary support and protection. Charter Medical recently developed a single-use frozen storage and transport shipping solution designed to complement single-use Freeze-Pakâ„¢ STS (FP-STS) biocontainers, which are engineered specifically for frozen storage applications. The bags and shippers…

Through the combined strengths of the New Brunswickâ„¢ and DASGIP® product portfolios, Eppendorf now has the product portfolio and expertise to provide an outstanding range of premium equipment and single-use solutions to meet all needs. A New Scale of Bioprocessing From the parallel mini bioreactor system DASbox for early stage bioprocess development to the benchtop and parallel bioreactor systems for laboratory scale and the sterilize-in-place solutions for production, Eppendorf offers industry and research users its extensive bioprocess solutions from a…

As major economies in the Asia–Pacific region continue to increase focus on healthcare modernization, new opportunities for both global and local biopharmaceutical companies are being created. Such opportunities are driving demand for local sourcing of raw materials to ensure security of supply. Modernization of Healthcare Drive Opportunities for Biopharmaceutical Firms Recent modernization of healthcare by governments has seen China pledge 124 billion to improve its healthcare system to provide insurance to 90% of its residents (1). South Korea introduced a…

To achieve good growth of microorganisms and high yields in protein expression, the following factors in fermentation are important: medium, gas Fermentors for research and development have to meet very high standards for flexibility so that they can be easily adapted to the requirements of a wide range of microorganisms and process conditions. For that purpose, modular fermentation systems were developed, consisting of single modules such as drive, fermentation vessel, analytical instrumentation, stirrer, foam separator, ventilation, and so on. This…