Catalent is your strategic partner for biologic drug development success. We create smart, tailored solutions with our novel SMARTag™ antibody–drug conjugation (ADC) technology, our market-leading GPEx® cell line expression system, expanded biomanufacturing capabilities, and integrated development services to help you get better products to market faster. New SMARTag ADC Technology for Development of Optimized Antibody–Drug Conjugates — Superior Technology for Treatments in Development: Catalent has entered into an exclusive license and comarketing agreement with Redwood Bioscience to offer customers SMARTag…

Figure 1:  Production of biologics — whether for clinical development or large-scale manufacturing campaigns intended to be converted to final drug product — often involves frozen storage. It provides manufacturing process flexibility while enabling long-term product stability. Products are frozen and stored using a number of technologies: stainless-steel vessels, bottles, carboys, and disposable bags. Although single-use freezing bags are a common choice for storing biologics, challenges with durability persist. Single-use bags intended for freezing and storage are often made with…

Although single-use bioreactors are widely applied in the biopharmaceutical industry, microbial cultivation in them is restricted due to limitations in oxygen mass transfer, mixing, and heat transfer. The CELL-tainer™ single-use bioreactor makes use of an innovative principle applying a two-dimensional (2D) rocking motion, thus providing excellent mass transfer, making this single-use reactor very suitable for microbial cultivation at different scales. With a simple pillow-shaped bag without moving parts, the technology is robust and leads to cost savings compared with other…

Setting its sights on the benefits to the customer, Eppendorf has steadily expanded its proven bioprocess technology portfolio since 2007. With the integration of New Brunswick™ and DASGIP®, Eppendorf combines its own expertise with the know-how of these renowned suppliers of bioreactors to set new benchmarks. For industrial and research users, this translates into sophisticated bioprocess software and hardware solutions from a single source that covers the full range from miniature bioreactor systems for R&D to production-scale, sterilize-in-place systems. The…

This application note describes the cultivation of Chinese hamster ovary (CHO) suspension cells in the Finesse SmartGlass vessel bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 × 106 cells/mL were achieved. Recombinant secreted embryonic alkaline phosphatase (SEAP) expression was induced by medium exchange and temperature shift. Maximum SEAP activities of up to 63 U/mL were reached. A novel stirred glass bioreactor suitable for cell culture applications at benchtop…

One of the major bottlenecks in the production of biopharmaceuticals is the efficient expression of therapeutic proteins in microbial or mammalian cells. The Escherichia coli pAVEway™ expression system described here has been developed to ensure high product titers and efficient scale up to GMP manufacture while minimizing many common issues seen in other expression systems, such as “leaky” expression (expression of recombinant protein in the absence of inducer). How It Works The use of a number of powerful E. coli…

Figure 1:  Figure 1:  When planning a modern 750-L fermentation plant including a cross-flow unit, the GEA Diessel company was looking for the best available measurement technology suited for its customer requirements. Attention turned to the new intelligent Arc sensors from Hamilton. After testing the Arc system, the plant engineer and GEA’s customer were convinced of the advantages of the new technology, deciding to use the Arc system in the fermentation plant. GEA Diessel and Hamilton were able to supply…

Table 1:&#8 The need for a rapid turn-around in quantity (and the loss of technicians in many laboratories) has created a trend toward single-use vessels. Their advantages include no time lost in cleaning or sterilization (especially for bench-scale, autoclavable systems), no chance of inhibition or cross-contamination due to poor cleaning, and no need for cleaning and sterilisation facilities in a crowded laboratory. For production-scale steel bioreactors, fully automated clean-in-place (CIP) and sterilization-in-place (SIP) has been possible for decades. By contrast,…

Researchers have turned to Chinese hamster ovary (CHO)–based transient gene expression (TGE) as an alternative to CHO stable cell line production for early stage antibody development. Despite advances in transfection methods and culture optimization, most CHO-based TGE systems produce antibody titers (low mg/L) that are insufficient for full use in biotherapeutic development pipelines. MaxCyte electroporation is a universal, transient transfection platform with unmatched quality, flexibility, and scalability and the capacity to transfect up to 2 × 1011 cells in under…

Disposable Clamps Qosina offers slide clamps, pinch clamps, and roller clamps in an assortment of sizes, colors, and styles. In stock and available for immediate delivery, these disposable clamps are available to fit tubing dimensions ranging from 0.06 inch OD to 0.75 inch OD. Material selection consists of polyacetal, polypropylene, polycarbonate, ABS, PVC, and many more to meet your needs. In addition, Qosina also carries clamps in a variety of sizes, styles, color, and materials, including closure clamps, catheter clamps,…