S-BIO1 has launched today EZGlyco™ mAb-N Kit with 2-AB, a breakthrough new product that enables scientists to analyze N -glycans in IgG and Fc-fusion proteins from culture supernatant samples in a rapid, simplified and streamlined single tube operation. The kit is a valuable tool supporting customers in glycoprotein characterization for biotherapeutic development.
Therapeutic monoclonal antibodies (IgG, mAb) are routinely expressed in recombinant expression systems. N-glycosylation is one of the most important post-translational modifications. Monitoring of glycosylation in antibody development and production directly from cell culture media will shorten the time needed to obtain the N-glycan profiling. HPLC-ready glycan preparation can be performed in less than 2 hours, allowing scientists to purify IgG from cell culture supernatant, release and label N-glycans in one complete kit. “The EZGlyco mAb-N Kit with 2-AB streamlined and rapid N-glycan preparation from IgG cell culture supernatant circumvents use of other kits for IgG purification and provides unmatched speed, ease of use, and compatibility with HPLC,” states Dr. Anju M. Dang, Director, S-BIO.
The new EZGlyco™ mAb-N Kit with 2-AB is currently available in the U.S. through S-BIO, Vaupell Holdings Inc., a Sumitomo Bakelite Co., Ltd. group company.