Author Archives: Ken Baker

Purification of a Monoclonal Antibody Using a Combination of UNOsphereâ„¢ Rapid S and UNOsphere Q Media Ion Exchange Chromatography After Protein A Capture

Figure 1. Antibody-based drugs represent a growing segment of the pharmaceutical industry and one of the most promising classes of therapeutic drugs. The demand for protein-based drugs is expected to grow steadily for the foreseeable future. However, production of monoclonal antibody (MAb)–based drugs remains very costly, and solutions to lower production costs are needed. Downstream purification steps are becoming a target for cost reduction. The use of ion-exchange chromatography to directly capture MAbs from cell culture streams or as a…