Author Archives: Jorg Schmidt Uwe Mueller Carla Wallimann Stephanie Mathes Claudia Probst Sandra Siegrist Carlo Andretta Christian Leist Ursula Graf-Hausner

Monitoring ATP Status in the Metabolism of Production Cell Lines

Development of industrial cell culture processes for production of recombinant proteins seeks high efficiency, reproducibility, and predictability. Usually the time allowed for process development is short, during which culture conditions and scale-up protocols must be defined so as to maximize cell productivity and yield while minimizing process scope and overall costs (1). Although scientific literature describes various methods that increase productivity of a cell culture by reducing and arresting cell growth or weakening cell physiology (2), the cells must be…