Author Archives: Helene Lafontaine

Designing a MAb Purification Process: Based on Bio-Sequential Chromatography

by

In batch-mode chromatography, operating conditions do not allow full use of the total media capacity (1, 2). Bio-Sequential Chromatography (BioSC®) is an open-loop process for the separation of multicomponent solutions (3). The unique column of the batch mode is replaced by a series of smaller columns (from two to six). This design maximizes the use of the media without product loss along the process sequences. The BioSC® Lab automated system is based on the multicolumn chromatography process, designed and provided…

August 10, 2016

Scaling Up a MAb Purification Process Using Continuous Chromatography

by

In batch-mode chromatography, operating conditions do not allow full use of a media’s total capacity, leading to a subutilization of the media (1, 2). Sequential multicolumn chromatography (SMCC) is an open-loop process for the separation of multicomponent mixture buffers (3, 4). The concept behind SMCC is to perform different steps in smaller columns (from two to eight columns, compared with batch chromatography) connected in series, to maximize media use without product loss. BioSC® Lab is an automated system based on…

August 18, 2015