Figure 1. Optimization of key factors in a biopharmaceutical process is necessary to meet the continuous rise in production demands. Choice of cell culture media and peptone supplementation is critical to process performance. However, medium development can be a multifaceted process requiring a large number of resources. To comprehensively cover all variables with the least time and effort, statistical methods are employed. Using statistical methods can help identify an animal free (AF) peptone that effectively enhances protein production for a…