Author Archives: Anna Heijbel

Polishing of Monoclonal Antibodies Using a Polymer Grafted Cation Exchanger

A typical monoclonal antibody purification process begins with a protein A capture step, followed by one or two polishing steps often using ion exchange chromatography media. This poster presents how to use the new cation exchange chromatography medium, Captoâ„¢ S ImpAct in MAb polishing. Capto S ImpAct is based on a newly developed grafting technology (Figure 1), resulting in high binding capacity and high resolution. To find the optimal conditions for static binding capacity of a MAb a screening was…