The determination of total capsid titer is one of the critical quality attributes for AAVs used in gene therapy. This application note presents quantitation of AAV serotype 2, 5, 8 and 9 using GatorPrime™ system and Gator™ AAVX probes, and compares the performance with Progen Xpress ELISA assay for the same serotypes.

The Progen ELISA kit is commonly used for AAV quantitation and while it performs well, it has several drawbacks for routine operation, viz, need to dilute the samples by 2 to 3 orders of magnitude to bring them into the ELISA working range. This has the potential to introduce large errors. Also, long analysis times and significant hands-on time. On the other hand, the Gator BLI solution has none of these constraints.

This application note demonstrates excellent accuracy and correlation with ELISA, and also highlights the benefits of the Gator BLI solution viz, ease of use, speed, wide dynamic range and minimal hands-on time.