Repligen™ OPUS (open Platform User Specified) prepacked columns packed with CaptivA™ PriMab™ Protein A resin offer high performance and all the advantages of single-use technology (reduced validation effort, reduced risk, faster time-to market). What makes this solution truly unique, however, is that oPUS prepacked columns provide lower total cost of ownership in many applications compared with conventional stainless steel or glass columns. Single-Use Chromatography Study BioPharm Services ltd (Chesham, UK) conducted a study to assess the impact on total cost…

          Irvine Scientific is committed to supporting the biopharmaceutical industry in the successful development and commercialization of biopharmaceutical products. A strong understanding of our media products is not the only basis for our successful supply, but the foundation of the innovation we provide to the market. Knowing innovation can result from the next big idea or an incremental change, we look for the small critical elements in our business that provide substantial process improvements and value…

LEWA developed its first pulseless pump for liquid chromatography in the late 1970s and delivered its first chromatography system in the early 1980s. LEWA is the leader in supplying pumps used for pilot and industrial HPlC, SMB, and lPlC chromatography worldwide. A customer came to us several years ago requesting a lowpressure liquid chromatography system with a flow range of 20 − 2,200 L/h. They also wanted the ability to achieve linear gradient from 1 to 99%. We designed and…

A subsidiary of the M + W Group, M+W Process Industries GmbH is a leading global engineering and construction company offering complete process and facilities solutions from design to construction. M+W Process Industries has the experience and the know-how for design and execution of all types of biotechnology and pharmaceutical production systems. The portfolio includes research plants as well as industrial facilities, monoproduct and multiproduct plants, variable single-use systems, and large-volume stainless-steel production units. The needs and requirements of our…

Continously new bioprocesses are evolving, and bioprocessors are searching for the easiest way to process samples in R&D. Currently, a huge trend towards disposable production systems is notable. For these purposes plastic bags, stirred-tank reactors, and storage tanks established on the market. Unfortunately, these reactor types are not suitable to perform sophisticated bioprocess development. In bioprocess R&D, throughputs of 50 to 1,000 fermentations are needed. Therefore, m2p-labs developed intelligent new tools at microscale to fulfill the needs in bioprocess development.…

          With a renewed acceptance of perfusion processesin cell in culture, both in the laboratory and for production applications (1), New Brunswick packed-bed bioreactors using Fibra-Cel® disks are seeing an upsurge in interest. This article examines Fibra-Cel technology and its many advantages, from increased yields of secreted proteins to labor savings in applications ranging from rabies vaccine production to production of the first licensed gene therapy drug.   What Is Fibra-Cel?   Fibra-Cel from New Brunswick…

          Advances in sensor materials and optoelectronics have enabled new optical sensors for use in biotechnology, food and beverage processing, and more. These fiber optic chemical sensors work by trapping an oxygen-sensitive fluorophore or pH indicator dye in a sol-gel host matrix that is applied to an adhesive patch, the tip of a fiber, or a flat substrate such as a cuvette. The indicator materials change optical properties in response to analytes in their environment, and…

    Fed-batch protein production processes have become more favored because of their simplicity under large-scale conditions. PAA laboratories′ ActiCHO media system is an industrial media package for the cultivation of Chinese Hamster Ovary (CHO) cells in large-scale applications. It contains all media needed from cell thaw until harvest in large scale, which are a stock culture and cell-expansion medium, a production medium, and two appropriate feed media. The formulation of each single medium is titrated carefully to complement the…

          In many biopharmaceutical companies, chromatographic purification in downstream processing is a key focus for optimization studies. The objective is to streamline the process, which may be achieved by the elimination of intermediate unit operations. The main question, therefore, is how to optimize the adjustment of individual chromatographic process steps. Currently there is a broad range of next-generation sorbents and membranes available for process chromatography. These new chromatography media are characterized by significantly improved performance compared…

Oligonucleotides are entering clinical trials in increasing numbers. In many ways, their entry into the clinic mirrors the development of protein therapeutics in the late 1980s and early 1990s. On the other hand, the purification process for an oligonucleotide therapeutic is very different from that for a protein. Typically one high-resolution, high-load step is used rather than a train of capture, intermediate purification, and polishing columns. TSKgel SuperQ-5PW (20) resin — a 20-µm particle size anion-exchange medium — is becoming…