In this work we present results with a novel high capacity Protein A medium prototype based on high flow agarose. The medium prototype was optimized for higher dynamic binding capacity (DBC) at long residence time. A 20–50% higher DBC was obtained compared to MabSelect SuReâ„¢. DBC was independent of MAb concentration in the range 1–10 g/L. Optimization of elution conditions (pH and flow rate) was performed by design of experiments. A lifetime study for 100 purification cycles, performed with a…
