Saturday, May 1, 2010 Daily Archives

Improving IEX Throughput and Performance with Differentiated Chromatography Sorbents

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As upstream processing becomes more optimized, it increases the amount of impurity and contaminants to be removed from large volumes of feedstock. With this added pressure on downstream processing, there is a pressing need for more efficient chromatography operations.

In this paper, the authors describe how they characterized differentiated selectivity of Q and S HyperCel ion-exchange sorbents. They evaluated the dynamic binding capacity in various pH, conductivity, and residence time conditions; carried out a separation of a model mixture to further characterize the sorbents’ specific selectivity; and report on a real case application in which one was used in a capture step for purification of a recombinant protein from an E. coli lysate.

May 1, 2010

Potelligent® CHOK1SV: The Evolution of the GS Gene Expression System™

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Lonza and BioWa have combined their technologies to produce a new host cell line, Potelligent® CHOK1SV. This new cell line merges the benefits of Lonza’s GS Gene Expression SystemTM with those of BioWa’s POTELLIGENT® technology. Recombinant cell lines created using this new and improved host cell line have shown:

• Enhanced ADCC
• Growth suitable for a production process
• High product concentration levels in a platform process
• Ability to work seamlessly with current manufacturing processes

Join Allison Porter of Lonza Biologics as she provides a detailed look at the two technologies separately and the performance of the new Potelligent® GS-CHO technology.

May 1, 2010

Improving Downstream Processing Using Membrane Chromatography

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The chromatographer is faced with a variety of anion and cation exchange phases that include a limited range of functional groups. These groups are attached to a wider range of base matrices, such as sorbents for column applications, monoliths and membranes. When ion-exchange membranes are used in chromatography devices, they demonstrate very high usable flow rates with enhanced throughput. Additionally the pre-packed format of these columns enables ease of use and unit-to-unit consistency. It also allows for a disposable chromatographic processes that eliminates costly and time-consuming cleaning validation.

During this on-demand webcast, diverse case studies will be presented that feature applications ranging from contaminant clearance to capture of target molecules in downstream purification processes. These studies highlight scalability, fast and efficient capture of large molecules, and improved process economics. When used in these applications, ion-exchange membranes proved a demonstrable alternative to packed bed chromatography and is better suited for subsequent purification processes..

Join Ajay R. Lajmi of Pall Life Sciences as he takes an in-depth look at the trends and challenges facing downstream processing and illustrates the benefits of membrane chromatography vs. resin chromatography.

May 1, 2010

Anatomy of a Single-Use Bioreactor Deployment

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Rapid commissioning and start-up enabled by system design and process support

Deploying a new GMP single-use bioreactor requires the careful coordination of hardware, software, disposable components, process engineering, tech-transfer and other disciplines. When time pressure is involved as it often is in the current business environment, the task of mastering an unfamiliar piece of process equipment can provide a challenge to biopharm organizations. Recognizing this, Xcellerex has designed the XDR single-use bioreactor as a fully-integrated system that is engineered for rapid delivery and start-up, capable of going from order to delivery to working process in just 12-16 weeks. Further, the Xcellerex team uses XDR reactors every day in our own GMP operations, allowing our technical team to provide fully informed support to customers. This webinar provides an overview of XDR deployment capabilities, and shares a recent customer case history of a successful rapid XDR deployment.

May 1, 2010